Enhanced Astrocyte Activity and Excitatory Synaptic Function in the Hippocampus of Pentylenetetrazole Kindling Model of Epilepsy

Simple item page
dc.contributor.authorDíaz, Franco
dc.contributor.authorAguilar, Freddy
dc.contributor.authorWellmann, Mario
dc.contributor.authorMartorell, Andrés
dc.contributor.authorGonzález-Arancibia, Camila
dc.contributor.authorChacana-Véliz, Lorena
dc.contributor.authorNegrón-Oyarzo, Ignacio
dc.contributor.authorChávez, Andrés E.
dc.contributor.authorFuenzalida, Marco
dc.contributor.authorNualart, Francisco
dc.contributor.authorSotomayor-Zárate, Ramón
dc.contributor.authorBonansco, Christian
dc.date.accessioned2024-02-19T17:46:05Z
dc.date.available2024-02-19T17:46:05Z
dc.date.issued2023-09-25
dc.description.abstractEpilepsy is a chronic condition characterized by recurrent spontaneous seizures. The interaction between astrocytes and neurons has been suggested to play a role in the abnormal neuronal activity observed in epilepsy. However, the exact way astrocytes influence neuronal activity in the epileptogenic brain remains unclear. Here, using the PTZ-induced kindling mouse model, we evaluated the interaction between astrocyte and synaptic function by measuring astrocytic Ca2+ activity, neuronal excitability, and the excitatory/inhibitory balance in the hippocampus. Compared to control mice, hippocampal slices from PTZ-kindled mice displayed an increase in glial fibrillary acidic protein (GFAP) levels and an abnormal pattern of intracellular Ca2+-oscillations, characterized by an increased frequency of prolonged spontaneous transients. PTZ-kindled hippocampal slices also showed an increase in the E/I ratio towards excitation, likely resulting from an augmented release probability of excitatory inputs without affecting inhibitory synapses. Notably, the alterations in the release probability seen in PTZ-kindled slices can be recovered by reducing astrocyte hyperactivity with the reversible toxin fluorocitrate. This suggests that astroglial hyper-reactivity enhances excitatory synaptic transmission, thereby impacting the E/I balance in the hippocampus. Altogether, our findings support the notion that abnormal astrocyte–neuron interactions are pivotal mechanisms in epileptogenesis.
dc.identifier.issn10.3390/ijms241914506
dc.identifier.other1422-0067
dc.identifier.urihttps://hdl.handle.net/20.500.12536/2117
dc.language.isoen
dc.sourceInternational Journal of Molecular Sciences
dc.subjectAstrogliosis
dc.subjectGliotransmission
dc.subjectExcitation–inhibition imbalance
dc.subjectRelease probability
dc.subjectPTZ-induced kindling
dc.subjectEpilepsy
dc.titleEnhanced Astrocyte Activity and Excitatory Synaptic Function in the Hippocampus of Pentylenetetrazole Kindling Model of Epilepsy
dc.typeArticle
uvm.carreraFonoaudiología
uvm.escuelaEscuela de Ciencias de la salud
uvm.indexWoS
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Artículo de revista - Enhanced Astrocyte Activity and Excitatory Synaptic Function in the Hippocampus of Pentylenetetrazole Kindling Model of Epilepsy.pdf
Size:
3.63 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos científicos