Protocol for in vitro rooting of Pyrus comunnis rootstocks

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dc.contributor.authorNadal, Michele Carla
dc.contributor.authorBernardes Machado, Otávio
dc.contributor.authorAraújo, Ronilson Carlos
dc.contributor.authorRodrigues, Filipe Almendagna
dc.contributor.authorRufato, Leo
dc.contributor.authorDória, Joyce
dc.contributor.authorPasqual, Moacir
dc.date.accessioned2023-07-14T14:36:52Z
dc.date.available2023-07-14T14:36:52Z
dc.date.issued2023-04-15
dc.description.abstractEffective protocols for in vitro rooting for woody fruit trees are still a challenge for in vitro seedling production, especially when there is a need to insert new cultivars or rootstocks. These protocols are essential to accelerate studies in plant breeding programs and for seedling distribution. This study evaluated the use of 6-Benzylaminopurine (IBA) in in vitro rooting of Pyruscomunnis rootstocks, clones ‘OHxF87’ and Pyrodwarf. Explant exposure times (0, 24, 48, 72, and 96 hours) to 20 mg L-1 IBA were tested for in vitro rooting. The exposure to IBA resulted in rooting rates above 80%, surpassing some results reported in the literature. The 24-hour treatment provided 81,81% survival, leading to an average growth of five roots with 19 mm length, for ‘OHxF87’ rootstock. The same exposure time resulted in the highest survival rate (75%) and the highest mean root number, seven roots per plant with 10 mm length, for ‘PDW’ rootstock. Root formation did not occur in the absence of synthetic auxin. Therefore, it can be concluded that a 24-hour exposure at 20 mg L-1 IBA was sufficient to promote in vitro rooting in ‘OHxF87’ and Pyrodwarf rootstocks’.es_ES
dc.identifier.issn1678-4596
dc.identifier.other10.1590/0103-8478cr20210661
dc.identifier.urihttps://hdl.handle.net/20.500.12536/1941
dc.language.isoenes_ES
dc.sourceCiência Rurales_ES
dc.subjectOHxF87es_ES
dc.subjectPDWes_ES
dc.subjectPlant tissue culturees_ES
dc.subjectPyrodwarfes_ES
dc.subjectSeedling productiones_ES
dc.subject6-Benzylaminopurinees_ES
dc.titleProtocol for in vitro rooting of Pyrus comunnis rootstockses_ES
dc.typeArtículo de revistaes_ES
uvm.escuelaEscuela de Ciencias Agrícolas y Veterinariases_ES
uvm.indexScopuses_ES
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