Browsing by Author "Petit-Breuilh, Ximena"

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    Hydrolyzed protein from Lupinus albus (INIA-Boroa variety) for the functional food industry: An example of partnership between farmers and academia
    (2023-01-04) Oliveira, Patricia E.; Benavides, Sergio F.; Huenufil, Daniel; Zarate, Pablina; Petit-Breuilh, Ximena
    This work presents investigations carried out to obtain by-products of bitter lupine (Lupinus albus), using a variety created specially in the Chilean Agricultural Research Institute (INIA). We present new information on obtaining protein isolates and hydrolysates as a way of adding value to bitter lupine, which promises to help return this crop to profitability in Chile. Lupine in this country is produced almost exclusively in the Araucanía Region, for the most part by small-scale farmers of the Mapuche ethnic group. An experimental design was drawn up based on one-factor experiments, for obtaining both protein isolate and protein hydrolysate. The best process conditions were achieved with stirring times of between 60 and 90 min; the concentrations (consistency of the mixture) were between 5 and 10%, with pH of the solution 12. The maximum yield achieved was 33% pure protein isolate (dry weight). After the protein isolates had been formulated, they were characterised by their functional properties: solubility, water retention capacity, lipid adsorption index, swelling capacity and foam stability. These properties are important for determining the quality of the protein hydrolysate, as they will affect its digestibility and thus its bioavailability in the organism. The protein hydrolysate was characterised by its complete proximal parameter, vitamins, total sugars, and amino acids. These results show that Lupinus albus (var. INIA-Boroa) is an excellent raw material for the existing functional foods industry.
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    Manufacture of a bio-tissue based on nanocrystalline cellulose from chilean bamboo Chusquea quila and a polymer matrix using electrospinning
    (Nano-Structures & Nano-Objects, 2020) Petit-Breuilh, Ximena; E.Díaz, Paola; Gacitúa, William; E.Oliveiraa, Patricia; E.Oliveira, Patricia
    Polymers of renewable origin have aroused great interest among researchers due to their sustainable, environmentally friendly nature. This work presents a biopolymer called bio-tissue, as it is created by electrospinning. This bio-tissue was formed with a polymer matrix of cellulose acetate (CA) reinforced with crystalline cellulose (NCC) derived from an autochthonous Chilean bamboo species of no commercial value which is considered a pest, Chusquea quila or quila. Bio-tissues were produced with three concentrations of NCC (1, 5 and 10% as a dry weight proportion of cellulose acetate) and their technological potential as an interior coating in small constructions was assessed. The morphology of the bio-tissues showed that the CA nanofibers containing NCC were of better quality and more uniform diameter than pure CA. The thermal profile showed that the highest concentration of NCC (10% as a dry weight proportion of cellulose acetate) induced earlier degradation; it was less thermally stable and decomposed at lower temperatures. The bio-tissue with 5% as a dry weight proportion of cellulose acetate showed the greatest resistance to traction with a break-point of 30 MPa and an elasticity module of 1.597 MPa. Finally, it was shown that the permeability to water vapour, oxygen and carbon dioxide was low, allowing regulated passage of these molecules. These results show the feasibility of using cellulose nanofibers as a reinforcement in polymer matrices; this could have various applications in fields ranging from the construction industry to food packaging.